Summary
Type: SPE
Application #: 304850
Catalog#: 730001 - SPE Cartridges, Chromabond, C18, 1ml, PP, 100mg, 100/pk
Detection:
Conditions: Column conditioning: 2 x 1 ml methanol, then 2 x 1 ml buffer (solution of 0.1% H3PO4 neutralized with NaOH to pH 7)
(Do not let run the column dry.)
Sample aspiration: An aliquot of 1000 µl of the prepared sample is passed through the column by vacuum (1-2 mm Hg)
Washing: 2 x 500 µl buffer (solution of 0.1% H3PO4 neutralized with NaOH to pH 7) by vacuum (6-7 mm Hg)
Drying: With vacuum (6-7 mm Hg, 5 min)
Elution: Analytes are eluted with 3 x 500 µl methanol
Evaporation: N2-stream, RT
Reconstitution: 100 µl MeOH/H2O (50:50)
Authors: SPE department
Source: Macherey-Nagel (Germany 2008)